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Related Experiment Video

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Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
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Temperature-responsive polymer-brush constructed on a glass substrate by atom transfer radical polymerization.

Hiromi Kitano1, Takuya Kondo, Hisatomo Suzuki

  • 1Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan. kitano@eng.u-toyama.ac.jp

Journal of Colloid and Interface Science
|March 9, 2010
PubMed
Summary

Temperature-responsive polymer brushes made of 2-(2-methoxyethoxy)ethyl methacrylate (MDM) exhibit controlled protein adsorption. These MDM polymer brushes show reduced protein adsorption below their lower critical solution temperature (LCST) and increased adsorption above it.

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Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

Published on: December 24, 2014

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biomaterials

Background:

  • Atom Transfer Radical Polymerization (ATRP) is a controlled polymerization technique.
  • Polymer brushes are polymer chains grafted to a surface.
  • Temperature-responsive polymers exhibit changes in solubility with temperature.

Purpose of the Study:

  • To synthesize and characterize a temperature-responsive polymer brush using 2-(2-methoxyethoxy)ethyl methacrylate (MDM).
  • To investigate the temperature-dependent behavior of the MDM polymer brush in aqueous solutions.
  • To evaluate the non-specific protein adsorption onto the MDM polymer brush surface at different temperatures relative to its lower critical solution temperature (LCST).

Main Methods:

  • Synthesis of MDM polymer brush via ATRP on a glass substrate.
  • Determination of the lower critical solution temperature (LCST) of the E-PMDM polymer.
  • Contact angle measurements to assess surface properties of the polymer brush.
  • Bicinchoninic acid assay to quantify protein adsorption (BSA, IgG, BPF).

Main Results:

  • The E-PMDM polymer exhibited an LCST of 26.2 °C.
  • The MDM polymer brush showed temperature-responsive changes in contact angle.
  • Significantly lower adsorption of IgG and BPF was observed below the LCST compared to above it.
  • BSA adsorption also demonstrated a temperature-responsive trend, albeit less pronounced.

Conclusions:

  • The synthesized MDM polymer brush exhibits tunable, temperature-dependent protein adsorption properties.
  • These findings suggest the potential of temperature-responsive polymer brushes for bio-medical applications requiring controlled surface interactions.
  • The pendent ω-methoxy oligo(ethylene glycol) groups contribute to the observed temperature-responsive behavior and antifouling characteristics.