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Updated: Jun 15, 2026

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

New technology for investigating trophoblast function.

¹Department of Perinatal Medicine Pregnancy Research Centre, Royal Women's Hospital, Parkville, 3052 Victoria, Australia. rosemary.keogh@thewomens.org.au

|March 9, 2010

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Continuous real-time monitoring of trophoblast cells using the xCELLigence System provides a comprehensive view of cell function. This technology allows for precise determination of growth phases, optimizing treatment and harvesting times for valuable insights.

Area of Science:

Cell biology
Reproductive science
Biotechnology

Background:

Trophoblast function is crucial for pregnancy success.
Current methods for measuring trophoblast function rely on endpoint assays, providing only a single time point measurement.
A need exists for real-time monitoring technologies to better understand dynamic cellular processes.

Purpose of the Study:

To introduce and evaluate the xCELLigence System for continuous, real-time monitoring of trophoblast cells.
To demonstrate the system's capability in quantifying key trophoblast functions like adhesion, proliferation, migration, and invasion.
To highlight the potential of this technology for optimizing cell culture and research at the maternal-fetal interface.

Main Methods:

Utilized the xCELLigence System with specialized micro-electrode culture dishes for real-time cell monitoring.

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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

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Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Related Experiment Videos

Last Updated: Jun 15, 2026

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Published on: January 8, 2012

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Measured cell adhesion, proliferation, migration, and invasion continuously over time.

Identified lag and log growth phases of trophoblast cells.

Main Results:

The xCELLigence System enabled continuous measurement and quantification of trophoblast cell behavior.
Real-time data provided a comprehensive picture of trophoblast function, surpassing single time-point assays.
Lag and log growth phases were accurately determined, indicating optimal intervention points.

Conclusions:

The xCELLigence System offers a powerful tool for real-time assessment of trophoblast function.
This technology provides valuable insights into cellular dynamics at the maternal-fetal interface.
Continuous monitoring enhances understanding and optimization of trophoblast cell behavior in research and potentially clinical applications.