Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Endospores and Sporulation01:20

Endospores and Sporulation

Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prophylactic intubation of the lacrimal duct in chemoradiotherapy of the lacrimal sac].

Die Ophthalmologie·2021
Same author

Effects of flowable liners on the shrinkage vectors of bulk-fill composites.

Clinical oral investigations·2021
Same author

New methodology to process shifted excitation Raman difference spectroscopy data: a case study of pollen classification.

Scientific reports·2020
Same author

Corrigendum to "Biochemical alterations in the Musculus triceps brachii and Musculus longissimus thoracis during early postmortem period in pigs" [Meat Science 152 (2019) 121-126].

Meat science·2019
Same author

Biochemical alterations in the Musculus triceps brachii and Musculus longissimus thoracis during early postmortem period in pigs.

Meat science·2019
Same author

Autofluorescence lifetime augmented reality as a means for real-time robotic surgery guidance in human patients.

Scientific reports·2019
Same journal

A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

Applied and environmental microbiology·2026
Same journal

Sources and traits of bacteria and fungi found in the near-surface atmosphere.

Applied and environmental microbiology·2026
Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026
Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026
Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026
Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
09:38

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

Published on: January 21, 2016

Raman spectroscopy-compatible inactivation method for pathogenic endospores.

S Stöckel1, W Schumacher, S Meisel

  • 1Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, Jena, Germany.

Applied and Environmental Microbiology
|March 9, 2010
PubMed
Summary
This summary is machine-generated.

A new inactivation method for Bacillus endospores allows for Raman spectroscopy analysis, enabling accurate identification of bacterial contamination in clinical and food settings. This technique aids in rapid detection and classification of microorganisms.

More Related Videos

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
10:03

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Related Experiment Videos

Last Updated: Jun 15, 2026

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
09:38

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

Published on: January 21, 2016

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
10:03

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

Published on: November 30, 2017

Area of Science:

  • Microbiology
  • Spectroscopy
  • Biochemistry

Background:

  • Micro-Raman spectroscopy offers rapid and sensitive detection of biological organisms.
  • Bacterial identification at species or subspecies levels is possible due to unique biochemical fingerprints.
  • Current limitations exist for Raman spectroscopy-compatible inactivation of resistant Bacillus endospores.

Purpose of the Study:

  • To develop and validate a Raman spectroscopy-compatible inactivation protocol for Bacillus endospores.
  • To enable species-specific identification of inactivated endospores using chemometric methods.
  • To assess the efficacy of inactivation and information preservation in Raman spectra.

Main Methods:

  • Evaluation of various physical and chemical inactivation methods for Bacillus endospores.
  • Application of 20% formaldehyde treatment as the superior inactivation method.
  • Utilizing chemometric techniques, including support vector machines and artificial neural networks, for data analysis.

Main Results:

  • 20% formaldehyde treatment demonstrated effective sporicidal capacity.
  • The inactivation protocol preserved crucial information in Raman spectra for identification.
  • Machine learning models successfully identified inactivated Bacillus anthracis-related endospores with adequate accuracy.

Conclusions:

  • A novel inactivation protocol for Bacillus endospores is presented, compatible with Micro-Raman spectroscopy.
  • This method facilitates reliable identification of bacterial species, crucial for contamination monitoring.
  • The developed protocol supports point-of-care testing and biosafety applications.