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Updated: Jun 15, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
[Highly sensitive method of detecting verotoxins produced by enterohaemorrhagic Escherichia coli O-157 : H7 using a
Rima Tanabe1, Byung-Yoon Cha, Takayuki Yonezawa
1Chubu University, Bioscience Research Center, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Abstract:
Enterohaemorrhagic Escherichia coli O-157 : H7 produces verotoxins called Shiga toxin or Shiga-like toxin. The type 1 and type 2 toxins present different immunological and physicochemical characteristics, and cause symptoms such as watery diarrhea, bloody diarrhea, severe stomach ache, fever and vomiting. The toxins can be assayed by several methods, including ELISA and PCR, with some limitations as to sensitivity. In the present study, we used a ProteoChip, which requires smaller volumes of reagents and allows detection of lower concentrations of the toxins, compared with the conventional assay.
