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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jun 15, 2026

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

[Selection of suitable polypropylene tubes for DNA testing using real-time PCR].

Eri Shimizu¹, Satoshi Futo, Tomoko Masubuchi

¹Fasmac Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.

Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan

|March 9, 2010

Summary

Selecting the right polypropylene microtubes is crucial for accurate DNA testing, as tube material can affect DNA binding and chemical elution. This study provides methods to identify optimal tubes for reliable bio-material and genetically modified organism (GMO) testing.

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Published on: March 21, 2018

Area of Science:

Molecular Biology
Biochemistry
Materials Science

Context:

Polypropylene microtubes are widely used for Polymerase Chain Reaction (PCR) and genetically modified organism (GMO) testing.
Tube selection is critical as material properties can influence DNA binding and chemical elution, potentially affecting test results.

Purpose:

To establish reliable methods for selecting polypropylene microtubes with optimal characteristics for DNA testing.
To address the variability in DNA testing outcomes caused by differences in microtube materials.

Summary:

Investigated the impact of polypropylene microtube material on DNA binding and chemical elution.
Developed and validated methods to assess and select microtubes suitable for sensitive DNA analysis.
Demonstrated that specific tube characteristics are essential for minimizing DNA loss and contamination.

Impact:

Provides a framework for researchers to choose appropriate microtubes, enhancing the accuracy and reproducibility of DNA-based assays.
Contributes to the standardization of pre-analytical steps in molecular diagnostics and genetic testing.
Aims to improve the reliability of results in fields relying on DNA analysis, including GMO detection and bio-material research.