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Related Concept Videos

Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...

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Related Experiment Video

Updated: Jun 15, 2026

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue
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Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Published on: June 18, 2014

TABLASER: trace (element) analyzer based on laser ablation and selectively excited radiation.

R M Measures, H S Kwong

    Applied Optics
    |March 9, 2010
    PubMed
    Summary

    Trace element analysis using laser ablation and selectively excited radiation (TABLASER) offers reliable in situ microultratrace quantification. This method shows promise for universal calibration curves, minimizing matrix effects for diverse samples.

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    Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue
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    Published on: June 18, 2014

    Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)
    09:05

    Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

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    Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
    09:22

    Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

    Published on: September 3, 2010

    Area of Science:

    • Analytical Chemistry
    • Atomic Spectroscopy
    • Laser-Induced Breakdown Spectroscopy (LIBS) related techniques

    Background:

    • Accurate trace element analysis is crucial for quality control in materials science and food safety.
    • Existing methods often suffer from matrix effects and limitations in sensitivity for in situ measurements.
    • A need exists for a reliable, quantitative microultratrace technique for in situ elemental analysis.

    Purpose of the Study:

    • To introduce and validate a novel technique, trace element analysis based on laser ablation and selectively excited radiation (TABLASER).
    • To assess the quantitative capabilities of TABLASER for in situ analysis of trace elements in various matrices.
    • To investigate the potential for reduced matrix effects and multielement analysis using TABLASER.

    Main Methods:

    • Utilized laser ablation to atomize sample material.
    • Employed selectively excited radiation (dye laser) for sensitive elemental detection.
    • Performed quantitative analysis on NBS standard reference steel, doped skim milk powder, and doped flour samples.

    Main Results:

    • Demonstrated a linear signal versus concentration dependence for chromium, extending beyond 1%.
    • Achieved current sensitivity limits in the parts per million (ppm) range for trace element analysis.
    • Observed relative freedom from chemical matrix effects, suggesting potential for universal calibration.

    Conclusions:

    • TABLASER is a promising new technique for reliable, quantitative in situ microultratrace element analysis.
    • Potential exists to improve sensitivity to the parts per billion (ppb) range with system optimization.
    • The technique's independence from matrix effects opens possibilities for universal calibration curves and multielement analysis.