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Mario Surbek1, Cemal Esen, Gustav Schweiger

  • 1Department of Mechanical Engineering, Ruhr-Universität Bochum, 44780 Bochum, Germany. surbek@lat.rub.de

Journal of Biophotonics
|March 9, 2010
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Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
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