Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence01:22

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Generic intravenous (IV) drugs are considered bioequivalent to their branded counterparts due to their 100% bioavailability upon administration. However, variations in stability among different drug products can significantly influence their therapeutic performance, even if they are pharmaceutically equivalent.Cefuroxime, a prophylactic antimicrobial, is often used as a single-dose IV injection for patients undergoing coronary artery bypass grafting surgery. A 3 g dose typically provides...
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry01:20

Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry

Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism01:21

Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism

Polymorphism refers to the existence of a drug substance in multiple crystalline forms, known as polymorphs. Recently, this term has been expanded to include solvates (forms containing a solvent), amorphous forms (non-crystalline forms), and desolvated solvates (forms from which the solvent has been removed).
Some polymorphic crystals possess lower aqueous solubility than their amorphous counterparts, leading to incomplete absorption. For instance, the oral suspension of Chloramphenicol, which...
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural Polymers in Tissue Engineering and Regeneration: Material-Cell Mechanotransduction, Biofabrication Strategies, and Clinical Translation.

Biomedicines·2026
Same author

Bioactive-Enriched Chitosan/Poly(vinyl Alcohol) Electrospun Nanofibers for Wound Healing: In Vitro and In Vivo Evaluation.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Achievements and Challenges in Therapy and Vaccines Development of Viral Hemorrhagic Fevers: An Up-to-Date Review.

Pharmaceutics·2026
Same author

Green-Synthesized Copper and Copper Oxide Nanoparticles: Structural Characterization and Evaluation of Biological Activity.

Antioxidants (Basel, Switzerland)·2026
Same author

Intranasal Drug Delivery in Neuropharmacology: Advances in Brain-Targeted Therapies and Bioethical Challenges.

Biomedicines·2026
Same author

Electrospun Chitosan-Poly(vinyl alcohol) Nanofibers Functionalized with Natural Bioactive Compounds: Design, Physicochemical Characterization and Release Profiles.

Polymers·2026
Same journal

Antioxidant Activity of Essential Oil From Carum Carvi L. Cultivated in North-Eastern Romania.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
Same journal

Assessment of In Vitro Antioxidant activity of Some New Ferulic Acid Derivatives.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
Same journal

Fast RP-HPLC Method for the Determination of Bisoprolol.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
Same journal

In Vitro Dissolution Studies of Amiodarone Hydrochloride From Hydroxy-Propyl-β-Cyclodextrin/Amiodarone Inclusion Complex Formulated Into Modified-Release Tablets.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
Same journal

Esthetic Rehabilitation Through CAD/CAM Technology - Case Report.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
Same journal

Correlation Among Chronological Age, Dental Age and Cervical Vertebrae Maturity in Romanian Subjects.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2018
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

[Erythromycin ethylsuccinate obtaining possibilities].

Cătălina Daniela Stan1, Alina Stefanache, Gladiola Tântaru

  • 1Disciplina de Industria Medicamentului si Biotehnologii Farmaceutice, Facultatea de Farmacie, Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi.

Revista Medico-Chirurgicala a Societatii De Medici Si Naturalisti Din Iasi
|March 10, 2010
PubMed
Summary
This summary is machine-generated.

This study improved erythromycin ethylsuccinate production by using water for crystallization, increasing yield to 82.02%. This method enhances purity and reduces costs compared to traditional solvents like hexane.

More Related Videos

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
05:46

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Related Experiment Videos

Last Updated: Jun 15, 2026

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
05:46

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Area of Science:

  • Pharmaceutical Chemistry
  • Organic Synthesis
  • Crystallization Techniques

Context:

  • Erythromycin ethylsuccinate is a crucial antibiotic.
  • Optimizing its synthesis and purification is vital for pharmaceutical production.
  • Traditional purification methods may have limitations in yield and cost-effectiveness.

Purpose:

  • To enhance the production process of erythromycin ethylsuccinate.
  • To investigate the efficacy of using water for crystallization as a purification step.
  • To improve the overall yield and purity of the final product.

Summary:

  • Erythromycin ethylsuccinate was synthesized via acylation and purified using a novel crystallization method in water.
  • The process involved acylation of erythromycin followed by crystallization in water at pH 8-8.5 and 5°C.
  • Quality control confirmed the product meets Romanian Pharmacopoeia standards, with a yield of 82.02% and microbiological activity of 98.7%.

Impact:

  • The use of water for crystallization significantly increases the process output (82.02%) compared to petroleum ether (74.14%) or hexane (80.25%).
  • This optimized method leads to reduced raw material consumption and lower production costs.
  • The enhanced purity and yield contribute to more efficient and economical manufacturing of erythromycin ethylsuccinate.