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[Determination of norisoboldine in Radix Lindera by RP-HPLC]
Jianzhong Chen1, Guixin Chou, Li Yang
1Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Objective:
To develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae.
Method:
The separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm.
Result:
The calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%.
Conclusion:
The developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.
