Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A stable perfluorochemical blood substitute.

B Mukherji1, H A Sloviter

  • 1Harrison Department of Surgical Research, University of Pennsylvania School of Medicine, Philadelphia.

Transfusion
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Indian Species of Artemesia.

The Indian medical gazette·2017
Same author

Indian Chenopodium.

The Indian medical gazette·2017
Same author

Bacillary Dysentery Treated with M.&.B. 693: A Case Report.

The Indian medical gazette·2017
Same author

High Altitude induced Ortner's Syndrome.

Medical journal, Armed Forces India·2016
Same author

In vivo staining and retardation of growth of sarcomata in mice.

The Anatomical record·2010
Same author

Selective action of certain dyestuffs on sarcomata and carcinomata.

The Anatomical record·2010

A stable perfluorodecalin emulsion, similar to blood viscosity, was developed. Tocopherol addition significantly reduced viscosity increases and lecithin oxidation during storage.

Area of Science:

  • Biomaterials Science
  • Colloid Chemistry
  • Biomedical Engineering

Background:

  • Development of stable emulsions is crucial for biomedical applications.
  • Perfluorodecalin (PFC) is a promising oxygen carrier, but its stability in emulsions needs improvement.
  • Egg yolk lecithin is a common emulsifier, but its oxidative stability can be a concern.

Purpose of the Study:

  • To develop a stable perfluorodecalin-in-water emulsion suitable for biomedical use.
  • To evaluate the long-term stability of the emulsion regarding viscosity and particle size.
  • To investigate the effect of tocopherol on the oxidative stability of the emulsion.

Main Methods:

  • A perfluorodecalin emulsion was prepared using sonication in Tyrode's buffer with egg yolk lecithin.

Related Experiment Videos

  • Viscosity and particle size were measured over storage at 5°C and 21°C.
  • Malondialdehyde accumulation was assessed to quantify lecithin oxidation.
  • The impact of adding tocopherol was evaluated.

    • Main Results:

    • A stable emulsion with 34% perfluorodecalin, blood-like viscosity, and 0.2-micron particle size was achieved.
    • Viscosity remained stable for 60 weeks at 5°C but increased after 20 weeks at 21°C.
    • Tocopherol addition significantly reduced viscosity increase at 21°C.
    • Tocopherol markedly reduced malondialdehyde formation, indicating decreased lecithin oxidation.

    Conclusions:

    • A stable, blood-compatible perfluorodecalin emulsion has been successfully developed.
    • Tocopherol acts as an effective antioxidant, enhancing the long-term stability of the emulsion.
    • This stable emulsion holds potential for applications requiring oxygen transport or as a blood substitute.