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Published on: November 7, 2018
[Determination of caryophyllene in Eupatorium fortune]
Dongjing Liu1, Xiaohong Zhao, Jian Xue
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China. liudongjing1101@sina.com
Summary
A new GC method accurately quantifies caryophyllene in Eupatorium fortune using ultrasonic extraction. This reliable method ensures quality control for E. fortune, a valuable medicinal plant.
Area of Science:
- Phytochemistry
- Analytical Chemistry
Background:
- Eupatorium fortune is a traditional medicinal herb.
- Caryophyllene is a key bioactive compound in E. fortune.
Purpose of the Study:
- To develop and validate a gas chromatography (GC) method for determining caryophyllene content in Eupatorium fortune.
- To establish a reliable quality control method for E. fortune.
Main Methods:
- Ultrasonic-assisted ethanol extraction was used to isolate caryophyllene.
- Gas chromatography with a DB-1701 quartz capillary column was employed for analysis.
Main Results:
- A linear calibration curve for caryophyllene was established (R² = 1) over the range of 0.002-2.0 g/L.
- The method demonstrated high recovery rates, ranging from 96.76% to 104.15%.
Conclusions:
- The developed GC method is accurate, simple, and exhibits good reproducibility.
- This validated method is suitable for routine quality control of Eupatorium fortune.
