Related Experiment Video
Updated: Jun 15, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Determination of chelerythrine in Chelidonium majus by RP-HPLC]
Objective:
To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.
Method:
Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.
Result:
The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.
Conclusion:
The method is accurate, sensitive and reliable.
Related Concept Videos
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Instrumentation
