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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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Expressed peptide assay for DNA detection.

Xin Zhou1, Peng Cao, Yuan Tian

  • 1Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210093, People's Republic of China.

Journal of the American Chemical Society
|March 10, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel nanoparticle-based assay for DNA detection using expressed peptide tags and mass spectrometry. This method overcomes limitations of current diagnostic strategies, enabling sensitive and multiplexed molecular identification.

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Area of Science:

  • Molecular Diagnostics
  • Nanotechnology
  • Mass Spectrometry

Background:

  • Existing molecular diagnostic platforms (planar/suspension arrays) face challenges with mass transport and limited multiplexing capabilities.
  • A need exists for innovative detection architectures that overcome these limitations for advanced molecular diagnostics.

Purpose of the Study:

  • To develop a novel expressed peptide assay using nanoparticle probes for DNA hybridization detection.
  • To demonstrate the capability of this assay for multiplexed DNA target identification via mass spectrometry.

Main Methods:

  • Utilized gold nanoparticle probes functionalized with peptide-encoding plasmids and oligonucleotides.
  • Employed microparticle probes with complementary oligonucleotides for target DNA capture.
  • Integrated magnetic separation and peptide expression for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification.

Main Results:

  • Successfully demonstrated the detection of two distinct DNA targets using unique peptide tags.
  • Confirmed the assay's modularity and applicability to protein targets, extending its diagnostic potential.
  • Highlighted the advantages of mass spectrometry's extensive mass tag options over existing systems.

Conclusions:

  • The proposed nanoparticle-based expressed peptide assay offers a distinct and modular approach to molecular diagnostics.
  • This method overcomes limitations of current technologies, enabling sensitive, multiplexed detection of DNA and other species.
  • The platform's design facilitates straightforward extension for broader diagnostic applications.