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Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

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Published on: April 7, 2023

Fluorescence lifetime in cardiovascular diagnostics.

Laura Marcu1

  • 1University of California, Davis, Department of Biomedical Engineering, Davis, California 95616, USA. lmarcu@ucdavis.edu

Journal of Biomedical Optics
|March 10, 2010
PubMed
Summary
This summary is machine-generated.

Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) and fluorescence lifetime imaging microscopy (FLIM) can diagnose atherosclerotic plaques. These fluorescence lifetime techniques identify biochemical features of plaque instability for improved cardiovascular disease diagnosis.

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Area of Science:

  • Biomedical optics
  • Medical diagnostics
  • Cardiovascular research

Background:

  • Atherosclerotic plaques pose a significant risk for cardiovascular events.
  • Current diagnostic methods for plaque instability have limitations.
  • Intrinsic fluorescence offers potential for label-free plaque characterization.

Purpose of the Study:

  • To review fluorescence lifetime techniques for atherosclerotic plaque diagnosis.
  • To assess the translational potential of TR-LIFS and FLIM.
  • To determine if intrinsic fluorescence provides diagnostic contrast for high-risk plaques.

Main Methods:

  • Review of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) instrumentation and methodologies.
  • Review of fluorescence lifetime imaging microscopy (FLIM) instrumentation and methodologies.
  • Analysis of intrinsic fluorescence signals for plaque biochemical feature discrimination.

Main Results:

  • TR-LIFS and FLIM enable characterization and diagnosis of atherosclerotic plaques.
  • Intrinsic fluorescence signals can provide diagnostic contrast for high-risk plaques.
  • These techniques discriminate biochemical features linked to plaque instability and rupture.

Conclusions:

  • Fluorescence lifetime techniques show promise for diagnosing atherosclerotic plaques.
  • TR-LIFS and FLIM have significant translational research potential.
  • These methods could be valuable for future intravascular diagnostic applications.