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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

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Fluorescence diffuse optical tomography for free-space and multifluorophore studies.

Anne Koenig¹, Lionel Hervé, Georges Gonon

¹CEA-LETI, Minatec, Grenoble, France. anne.koenig@cea.fr

Journal of Biomedical Optics

|March 10, 2010

Summary

We enhanced a preclinical fluorescence-enhanced diffuse optical tomography (fDOT) system for improved small animal imaging. This advanced fDOT system allows for experiments without adaptation liquid or glass plates, enabling dual fluorophore discrimination.

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Area of Science:

Biomedical Imaging
Optical Tomography
Preclinical Research

Background:

Diffuse optical tomography (DOT) is a valuable tool for preclinical imaging.
Existing DOT systems often require specific adaptations for animal studies.
Distinguishing multiple fluorophores in vivo presents a significant challenge.

Purpose of the Study:

To introduce and evaluate major advancements in a preclinical fluorescence-enhanced diffuse optical tomography (fDOT) system.
To demonstrate the system's capability for simplified in vivo experiments.
To showcase the ability to differentiate between two injected fluorophores.

Main Methods:

Development of an fDOT system with enhanced capabilities.
Implementation of a filter wheel for spectral discrimination of fluorophores.
Performance evaluation using characterization phantoms and in vivo mouse models.

Main Results:

The enhanced fDOT system enables experiments without the need for adaptation liquid or a glass plate.
The integrated filter wheel successfully discriminates between two injected fluorophores.
System evaluation on phantoms and in vivo demonstrated its utility for small animal biological studies.

Conclusions:

The presented fDOT system offers significant improvements for preclinical fluorescence imaging.
The system's enhanced features simplify experimental procedures and expand its applicability.
This advanced fDOT technology enriches biological studies on small animals.