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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 15, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Angular domain fluorescence imaging for small animal research.

Fartash Vasefi1, Michelle Belton, Bozena Kaminska

  • 1Simon Fraser University, The School of Engineering Science, Burnaby, British Columbia, Canada.

Journal of Biomedical Optics
|March 10, 2010
PubMed
Summary
This summary is machine-generated.

Angular Domain Fluorescence Imaging (ADFI) enhances detection of deep-tissue fluorophores in small animals. This novel technique offers superior resolution and contrast for biomedical imaging applications.

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Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Fluorescence Imaging with One-nanometer Accuracy (FIONA)

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Related Experiment Videos

Last Updated: Jun 15, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Area of Science:

  • Biomedical Optics
  • Fluorescence Imaging
  • Medical Physics

Background:

  • Detecting fluorophores deep within biological tissues is challenging due to light scattering.
  • Conventional imaging systems often struggle with resolution and contrast for subsurface targets.

Purpose of the Study:

  • To develop and evaluate a novel Angular Domain Fluorescence Imaging (ADFI) system.
  • To assess ADFI's efficacy for near-infrared fluorescent marker imaging in small animal models.

Main Methods:

  • Utilized an Angular Filter Array (AFA) for collimation detection, enabling acceptance angle filtration.
  • Developed an ADFI system for macroscopic imaging of fluorescent markers.
  • Applied the technique to image fluorescent bone markers in hairless mice.

Main Results:

  • ADFI demonstrated higher resolution and contrast compared to conventional lens and lens-pinhole systems.
  • ADFI successfully visualized vertebral structural and morphometric data in mice.
  • Results showed good correlation with volumetric X-ray computed tomography data.

Conclusions:

  • ADFI is a promising technique for imaging fluorophores embedded in biological tissues.
  • The developed ADFI system is effective for submillimeter mapping of fluorescent biomarkers in small animals.
  • ADFI offers significant advantages for preclinical biomedical research and diagnostics.