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10:09
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
Published on: April 28, 2011
Thermal-assisted refolding: dilution folding initiated at high temperature
Kentaro Shiraki1, Soichiro Kayano
1Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan. shiraki@bk.tsukuba.ac.jp
Current Pharmaceutical Biotechnology
|March 10, 2010
Abstract:
Protein refolding from unfolded state is usually carried out at low temperature to reduce protein aggregation and proteolytic degradation. This review briefly introduces a unique method for the protein refolding via high temperature, typically at above melting temperature of the protein.
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