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Updated: Jun 15, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

Step-wise refolding of recombinant proteins

Kouhei Tsumoto1, Tsutomu Arakawa, Linda Chen

  • 1Department of Medical Genome Sciences, Graduate School of Engineering, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan. tsumoto@k.u-tokyo.ac.jp

Current Pharmaceutical Biotechnology
|March 10, 2010
PubMed
Summary

Protein refolding is challenging. This study introduces step-wise dialysis refolding, a method controlling protein folding pathways by adjusting denaturant concentrations for sequential folding and disulfide bond formation.

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