Step-wise refolding of recombinant proteins
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Updated: Jun 15, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Kouhei Tsumoto1, Tsutomu Arakawa, Linda Chen
1Department of Medical Genome Sciences, Graduate School of Engineering, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan. tsumoto@k.u-tokyo.ac.jp
Abstract:
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
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