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Updated: Jun 15, 2026
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
Development of an insect cell-free system
Toru Ezure1, Takashi Suzuki, Masamitsu Shikata
1Clinical and Biotechnology Business Unit, Life Science Business Department, Analytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. ezure@shimadzu.co.jp
Abstract:
Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out post-translational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.
