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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Published on: August 20, 2018
Silkworm as a host of baculovirus expression
Akihiro Usami1, Takeo Suzuki, Hidekazu Nagaya
1Research Institute of Biological Science, Katakura Industries, Co., Ltd., 1548 Shimo-okudomi, Sayama, Saitama 350-1332, Japan. a.usami@katakura.co.jp
Current Pharmaceutical Biotechnology
|March 10, 2010
Summary
The silkworm expression system, an alternative to insect cell lines, offers robust high expression of recombinant proteins. Technology advancements enhance virus recombination and protein solubility, with potential for further improvements.
Area of Science:
- Biotechnology
- Molecular Biology
- Protein Expression Systems
Background:
- The Baculovirus Expression Vector System (BEVS) primarily utilizes insect cell lines like Sf9.
- A robust high expression system using silkworms has been developed as an alternative.
- Further technological improvements are needed for optimal protein production.
Purpose of the Study:
- To review advancements in silkworm expression technology.
- To highlight the advantages of using silkworms for recombinant protein production.
- To discuss example applications and areas for future development.
Main Methods:
- Improvements in virus recombination efficiency.
- Strategies to reduce proteolytic degradation and protein aggregation.
- Development of more reliable promoter systems for enhanced expression.
Main Results:
- Achieved high and soluble expression of recombinant proteins.
- Demonstrated the effectiveness of the improved silkworm expression system.
- Provided examples of successful protein production applications.
Conclusions:
- Silkworm expression systems offer a powerful alternative to traditional insect cell lines.
- Ongoing technological enhancements continue to improve protein yield and quality.
- Further research can unlock the full potential of this expression platform.
