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Updated: Jun 15, 2026

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
Published on: August 14, 2021
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy
Claudia Di Blasi1, Flavia Blasevich, Emanuela Bellafiore
1Neuromuscular Diseases and Neuroimmunology Unit, C. Besta National Neurological and Foundation, Via Temolo 4, 20126 Milan, Italy.
Abstract:
We report on a patient with muscle pain not associated with muscle weakness. Microscopic examination of the muscle biopsy revealed rod-like cytoplasmic bodies in many fast fibres. By electron microscopy these had a crystalloid structure identical to the hexagonally cross-linked caveolin 3-positive tubular arrays, previously described in patients with similarly benign myopathy. We found that these inclusions were positive for calsequestrin and for the calsequestrin-binding protein junctin, as well as for caveolin 3. However, the genes coding for these proteins were not mutated. For diagnostic purposes calsequestrin and caveolin 3 positivity should be checked when rods are encountered in muscle biopsy for mild myopathy.
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