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DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...

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Related Experiment Video

Updated: Jun 15, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Transposon transgenesis in Xenopus.

Donald A Yergeau¹, Clair M Kelley, Haiqing Zhu

¹Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.

Methods (San Diego, Calif.)

|March 10, 2010

Summary

Transposon transgenesis in Xenopus provides a straightforward method for creating germline transgenic animals. This technique utilizes micro-injection of embryos, simplifying the generation of genetically modified frogs for research.

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Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

Transposon-mediated integration offers efficient gene delivery in various organisms.
Xenopus laevis is a widely used model organism for developmental studies.

Purpose of the Study:

To provide a detailed, step-by-step guide for transposon-mediated transgenesis in Xenopus.
To outline methods for screening germline transmission and managing transgenic Xenopus populations.

Main Methods:

Co-injection of fertilized Xenopus embryos with transposon DNA and transposase mRNA.
Micro-injection technique suitable for routine laboratory use.
Screening methods for identifying mosaic founders with germline transmission.

Main Results:

Successful generation of germline transgenic Xenopus animals using transposon transgenesis.
Mosaic integration of transgenes at early developmental stages.
Established protocols for screening and husbandry of transgenic Xenopus.

Conclusions:

Transposon transgenesis is a simple, robust, and accessible method for generating transgenic Xenopus.
This technique facilitates the study of gene function in a key developmental model organism.
The provided guide aids researchers in establishing and managing transgenic Xenopus lines.