Functional genomics and genetical genomics approaches towards elucidating networks of genes affecting meat...

The benefit of functional genomics is to identify key pathways and functional networks of genes and candidate genes underlying the genetic control of phenotypes. Genetical genomics, i.e. the integration of genetic analysis and expression phenotypes, has the potential to uncover regulatory networks controlling the coordinated expression of genes and to map variation on the level of DNA affecting the mRNA expression. Here we illustrate our own attempts to apply functional genomics and genetical genomics approaches in order to identify functional networks of genes relevant to traits related to meat performance. Expression data of 74 M longissimus dorsi samples obtained using Affymetrix GeneChips were correlated with drip loss and principal components (PCs) with high loadings of meat quality traits. Functional annotation analyses revealed that differences in water holding capacity, early pH decline and ultimate pH were related to the ubiquitin-proteasome system, mitochondrial metabolic pathways and muscle structural aspects. In particular, 1279 genes were correlated with drip loss (P