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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Related Experiment Video

Updated: Jun 15, 2026

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Automatic fluorescence microphotometer using an image dissector tube.

S Wungkobkiat, T Morita, T Uesaka

|March 10, 2010

Summary

This summary is machine-generated.

This study introduces an automated fluorescence microphotometer for precise cell analysis. The system accurately measures fluorescence intensity and generates histograms for biological samples like rat liver nuclei.

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Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

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Last Updated: Jun 15, 2026

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

Area of Science:

Biomedical Engineering
Cell Biology
Analytical Chemistry

Background:

Accurate measurement of cellular fluorescence is crucial for quantitative biological analysis.
Traditional methods can be labor-intensive and prone to variability.
Developing automated systems enhances precision and efficiency in fluorescence microscopy.

Purpose of the Study:

To describe an automated fluorescence microphotometer system.
To enable precise measurement of weak fluorescent signals from biological samples.
To automate cell detection, fluorescence intensity measurement, and data presentation.

Main Methods:

Utilized an image dissector tube in photon-counting mode for sensitive fluorescence detection.
Integrated a laboratory-built microcomputer system for automated cell searching and positioning.
Employed raster scanning for fluorescence intensity mapping.
Applied Feulgen staining to measure DNA in rat liver nuclei for system validation.

Main Results:

Demonstrated precise intensity measurements of weak fluorescent spots.
Successfully automated the process of cell detection and fluorescence measurement.
Generated fluorescence distribution histograms of stained cells.
Validated system performance using DNA measurements in rat liver nuclei.

Conclusions:

The developed automatic fluorescence microphotometer offers precise and automated quantitative analysis of cellular fluorescence.
The system's capabilities include sensitive detection, automated sample handling, and comprehensive data presentation.
This instrument is valuable for research requiring accurate fluorescence measurements in biological samples.