Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible translocation of acyl-CoA:cholesterol acyltransferase (ACAT) between the endoplasmic reticulum and vesicular structures.

Frontiers in molecular biosciences·2023
Same author

OXTR-Related Markers in Clinical Depression: a Longitudinal Case-Control Psychotherapy Study.

Journal of molecular neuroscience : MN·2021
Same author

Sodium functions as a negative allosteric modulator of the oxytocin receptor.

Biochimica et biophysica acta. Biomembranes·2018
Same author

Synthesis and characterization of a novel rhodamine labeled cholesterol reporter.

Biochimica et biophysica acta. Biomembranes·2017
Same author

Interaction of G protein coupled receptors and cholesterol.

Chemistry and physics of lipids·2016
Same author

Eimeria bovis infection modulates endothelial host cell cholesterol metabolism for successful replication.

Veterinary research·2015

Related Experiment Video

Updated: Jun 15, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

Cholesterol-protein interaction: methods and cholesterol reporter molecules.

Gerald Gimpl1

  • 1Institut für Biochemie, Johannes Gutenberg-Universität, Johann-Joachim-Becherweg 30, Mainz, Germany. Gimpl@uni-mainz.de

Sub-Cellular Biochemistry
|March 10, 2010
PubMed
Summary

This study details methods for investigating cholesterol-protein interactions in cell membranes. It explores cholesterol probes and their application in understanding protein localization within cholesterol-rich microdomains.

More Related Videos

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Related Experiment Videos

Last Updated: Jun 15, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Cholesterol is vital for eukaryotic plasma membrane structure and function.
  • It influences phospholipid bilayer fluidity and membrane microdomain formation.
  • Cholesterol is a precursor for steroid hormones and bile acids.

Purpose of the Study:

  • To describe methods for exploring protein binding and interactions with cholesterol.
  • To examine the localization of membrane proteins in cholesterol-rich domains.
  • To investigate the specificity of cholesterol-protein interactions.

Main Methods:

  • Utilizing various cholesterol probes (radio-, spin-, photoaffinity-, fluorescent labels).
  • Identifying known cholesterol-binding molecules like polyenes and cytolysins.
  • Analyzing candidate membrane proteins for localization in cholesterol-rich microdomains.

Main Results:

  • Availability of diverse cholesterol probes for interaction studies.
  • Examples of established cholesterol-binding molecules provided.
  • Focus on specificity and localization aspects of cholesterol-protein interactions.

Conclusions:

  • Cholesterol-protein interactions are critical for membrane organization and function.
  • Various labeling techniques enable detailed study of these interactions.
  • Understanding these interactions aids in elucidating cellular processes.