Related Experiment Video

Updated: Jun 15, 2026

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

ATM is involved in cell-cycle control through the regulation of retinoblastoma protein phosphorylation

Javier G Pizarro1, Jaume Folch, Aurelio Vazquez de la Torre

  • 1Unitat de Farmacologia i Farmacognosia, Institut de Biomedicina (IBUB), Centro deInvestigacion Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), Facultat de Farmacia, Universitat de Barcelona, Nucli Universitari de Pedralbes, Barcelona E-08028, Spain.

Journal of Cellular Biochemistry
|March 10, 2010
PubMed

Insights

Ataxia telangiectasia mutated protein (ATM) regulates cell cycle control by modulating retinoblastoma protein (pRb) phosphorylation. This study reveals a novel ATM molecular network impacting cell cycle progression and DNA repair pathways.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Neuroscience

Background:

  • Ataxia telangiectasia mutated (ATM) protein, a PI3K family member, is crucial for DNA double-strand break (DSB) response.
  • Understanding ATM's role in cell-cycle regulation is vital for cellular health and disease research.

Purpose of the Study:

  • To investigate the function of ATM in cell-cycle control within dopaminergic rat neuroblastoma B65 cells.
  • To elucidate the molecular mechanisms by which ATM influences cell-cycle regulators.

Main Methods:

  • Pharmacological inhibition of ATM using KU-55933.
  • Partial gene silencing of ATM via small interfering RNA (siRNA) transfection.
  • Analysis of cell-cycle proteins, including cyclin A, retinoblastoma protein (pRb), and p53.

Main Results:

  • ATM inhibition or silencing reduced levels of cyclin A and phosphorylated pRb (Ser780).
  • ATM modulation decreased active p53 (Ser15 phosphorylation), Bax, and p21 expression.
  • ATM inhibition prevented H(2)O(2)-induced pRb phosphorylation, confirming its role in DSB response.

Conclusions:

  • ATM plays a significant role in regulating the cell cycle through the control of pRb phosphorylation.
  • A novel molecular network involving ATM in cell-cycle regulation has been identified.
  • ATM represents a potential therapeutic target for conditions involving cell-cycle dysregulation.

More Related Videos

Analysis of Cell Cycle Position in Mammalian Cells12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...