Related Experiment Video
Updated: Jun 15, 2026
07:10
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
Published on: April 21, 2019
Cross-sectional survey on immunoglobulin E reactivity in 23,077 subjects using an allergenic molecule-based
E Scala1, C Alessandri, M L Bernardi
1Centre for Clinical and Experimental Allergology, IDI-IRCCS, Rome, Italy.
Summary
This study evaluated IgE reactivity to 75 allergenic molecules in Italian patients. High prevalence of sensitization was observed, with distinct patterns emerging based on age and allergen type, aiding allergy diagnosis.
Area of Science:
- Immunology
- Allergology
- Molecular Diagnostics
Background:
- High-throughput technologies enable simultaneous IgE testing for multiple allergens.
- Microarray systems facilitate routine diagnostic work-up for allergic diseases.
Purpose of the Study:
- To conduct a cross-sectional evaluation of IgE reactivity prevalence to allergenic molecules.
- To assess sensitization patterns in a large Italian patient cohort using an innovative tool.
Main Methods:
- Utilized the ISAC microarray system for specific IgE detection.
- Analyzed serum samples from 23,077 individuals with allergic complaints.
- Tested reactivity against 75 distinct allergenic molecules.
Main Results:
- Over 70% of patients (16,408/23,077) showed IgE reactivity to at least one allergen.
- Cup a 1, Der f 2, and Phl p 1 were the most prevalent sensitizers.
- Sensitization prevalence varied significantly by age, revealing distinct lifetime sensitization patterns.
Conclusions:
- Allergen-based microarrays provide precise and comprehensive IgE sensitization evaluation.
- This approach enhances IgE-based epidemiology and understanding of sensitization processes.
- The findings support the use of microarrays for detailed allergy diagnostics.
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The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.