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Published on: April 17, 2020
Molecular characterization of VP7 gene of human rotaviruses from Bangladesh
Kamruddin Ahmed1, Selim Ahmed, Marcelo Takahiro Mitui
1Division of Infectious Diseases, Department of Social and Environmental Medicine, Institute of Scientific Research, Oita University, Yufu, Oita, Japan. ahmed@med.oita-u.ac.jp
Insights
Rotavirus diarrhea affected 39.5% of Bangladeshi children under five. Dominant G2 strains showed diversity and genetic links to Indian rotaviruses, highlighting ongoing strain evolution.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Molecular Epidemiology
Background:
- Rotavirus is a leading cause of severe diarrheal disease in children globally.
- Understanding circulating rotavirus strains is crucial for vaccine efficacy and public health strategies.
Purpose of the Study:
- To characterize rotavirus strains, including G and P genotypes, in children under five in Bangladesh.
- To investigate the phylogenetic relationships of these strains with global counterparts.
Main Methods:
- Retrospective analysis of rotavirus-positive stool samples from children under five.
- G and P genotyping using molecular methods.
- Phylogenetic analysis of VP7 gene sequences.
Main Results:
- Rotavirus diarrhea prevalence was 39.5%.
- Dominant genotypes were G2 (45.5%), G1 (24.8%), G12 (9.6%), G9 (8.5%), and G4 (2.1%).
- Specific G-P type combinations were observed, and Bangladeshi strains showed genetic relatedness to Indian strains, with some G9 strains clustering with those from Belgium, Australia, Sri Lanka, and Turkey.
Conclusions:
- Diverse rotavirus strains, including common and emerging genotypes, circulated in Bangladeshi children.
- Genetic diversity was evident in G and P type combinations and phylogenetic analysis.
- Amino acid substitutions in VP7 suggest ongoing viral evolution and potential implications for immunity.
Abstract:
This study was carried out during July 2005-June 2006, to characterize rotaviruses circulating in Bangladeshi children less than 5 years attended a peri-urban hospital. The proportion of rotavirus diarrhea was 39.5%. Genotype G2 was dominant (45.5%) followed by G1 (24.8%), G12 (9.6%), G9 (8.5%), and G4 (2.1%). G2 were mainly in combination with P[4], G1 and G9 with P[8], and G12 with P[6]. Phylogenetically Bangladeshi G1, G2, and G12 were closely related with the respective types from India, whereas Bangladeshi G9s of lineage III were with strains from Belgium and Australia. A G9 strain of lineage IV was clustered with strains from Sri Lanka and Turkey. Compared with prototype rotaviruses, Bangladeshi strains showed several amino acid substitutions at the antigenic sites of VP7. This study showed that the generation of diverse strains continued as evidenced by long G2, short G1 and G9 strains, and various combinations of G and P types.
