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Related Experiment Video

Updated: Jun 15, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

New methods for reverse transfection with siRNA from a solid surface.

Satoshi Fujita1, Kota Takano, Eiji Ota

  • 1Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2010
PubMed
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We developed two cost-effective reverse transfection methods for small interfering RNA (siRNA) on solid surfaces, enabling high-throughput functional gene analysis. These techniques are efficient for gene knockdown and phenotypic screening in cell cultures.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Reverse transfection offers advantages over traditional forward transfection for certain applications.
  • Developing efficient and scalable methods for reverse transfection is crucial for high-throughput screening.

Purpose of the Study:

  • To present two novel, inexpensive methods for reverse transfection using small interfering RNA (siRNA) from solid surfaces.
  • To optimize and validate these methods for efficiency, cytotoxicity, and gene knockdown in cell lines, focusing on HeLa cells.

Main Methods:

  • Localized reverse transfection on glass slides to create transfection microarrays (TMAs).
  • Reverse transfection in multi-well microtiter plates.
  • Evaluation of transfection efficiency, cytotoxicity (using Calcein-AM fluorescence), and gene knockdown efficacy.

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Related Experiment Videos

Last Updated: Jun 15, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
15:49

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
13:27

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

Main Results:

  • Established optimized conditions for cell culture, reagent preparation, and reverse transfection for HeLa cells.
  • Demonstrated efficient gene knockdown and minimal cytotoxicity with the developed methods.
  • Successfully performed phenotypic screening for a functional gene using the reverse transfection approach.

Conclusions:

  • The described methods provide powerful, cost-effective tools for high-throughput functional gene analysis.
  • These techniques facilitate efficient siRNA delivery for gene knockdown and phenotypic screening.
  • The developed reverse transfection protocols are adaptable for various cell lines and screening applications.