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Updated: Jun 15, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
Determination of alternate splicing events using the Affymetrix Exon 1.0 ST arrays
Sita Subbaram1, Marcy Kuentzel, David Frank
1Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY, USA.
Abstract:
Alternative splicing plays an important role in regulation of normal cellular function. Alternative splicing of pre-mRNA leads to the diversity of downstream protein products in the cell. The Affymetrix Exon arrays allow for a high throughput evaluation of the differences in spliced mRNA expressed in a biological system. In this study, we describe a method using this technology to study the generation of alternative mRNA transcripts in breast cancer cells that differ in the levels of a particular integrin, alpha3beta1.
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