A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon
Adam J Kimple1, Robin E Muller, David P Siderovski
1Department of Pharmacology, The University of North Carolina, Chapel Hill, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2010
Abstract:
Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.

