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[Corticosteroids determination in pig plasma (author's transl)].
Annales De Recherches Veterinaires. Annals of Veterinary Research
|January 1, 1977
Summary
Comparing two methods for measuring corticosteroid levels in pigs, researchers found both accurate for assessing adrenal function. The competitive protein-binding assay is suitable for routine analysis despite lower specificity.
Area of Science:
- Veterinary Endocrinology
- Biochemical Assays
Background:
- Accurate measurement of corticosteroid levels is crucial for assessing adrenal function in pigs.
- Existing methods for corticosteroid analysis vary in specificity and practicality.
Purpose of the Study:
- To compare the accuracy and precision of the competitive protein-binding assay (CPBA) with fluorimetric determination for measuring corticosteroid levels in pigs.
- To evaluate the suitability of CPBA for routine analysis of adrenal function in swine.
Main Methods:
- Peripheral blood samples from pigs were analyzed using Murphy's competitive protein-binding assay.
- Cortisol and corticosterone levels were determined by fluorimetric analysis after separation via thin-layer chromatography.
- Assay accuracy, precision, and sensitivity were evaluated.
Main Results:
- Corticosteroid levels measured by CPBA were 3.4 ± 0.3 µg/100 ml.
- Fluorimetric determination yielded 2.4 ± 0.2 µg/100 ml cortisol and 0.3 ± 0.03 µg/100 ml corticosterone.
- Both methods demonstrated sensitivity to diurnal variations, ACTH administration, and dexamethasone treatment.
Conclusions:
- Both competitive protein-binding assay and fluorimetric determination are sensitive and accurate for assessing adrenal function in pigs.
- The competitive protein-binding assay, despite its relative lack of specificity, is suitable for routine corticosteroid analysis in swine.