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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Updated: Jun 15, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Profiling DNA methylomes from microarray to genome-scale sequencing.

Yi-Wei Huang1, Tim H-M Huang, Li-Shu Wang

  • 1The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA. Yi-wen.huang@osumc.edu

Technology in Cancer Research & Treatment
|March 12, 2010
PubMed
Summary
This summary is machine-generated.

This review covers DNA methylation profiling technologies, including microarrays and next-generation sequencing. Advances enable single-base resolution mapping, offering new insights into genome regulation and dynamics.

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Last Updated: Jun 15, 2026

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • DNA cytosine methylation is a key epigenetic modification.
  • It plays critical roles in genome regulation, development, and disease.

Purpose of the Study:

  • To review current and emerging technologies for DNA methylation profiling.
  • To discuss the applications and limitations of different methodologies.
  • To highlight advances in next-generation sequencing for methylome analysis.

Main Methods:

  • Review of microarray-based technologies.
  • Review of next-generation sequencing (NGS) based technologies.
  • Discussion of combined approaches for comprehensive methylome analysis.

Main Results:

  • Microarray and NGS technologies offer distinct applications and limitations for DNA methylation profiling.
  • NGS advances enable global DNA methylation mapping at single-base resolution.
  • Combining multiple technologies can provide a more complete methylome picture.

Conclusions:

  • Technological advancements are crucial for understanding DNA methylation.
  • Single-base resolution mapping provides deeper insights into epigenetic regulation.
  • Future research will benefit from integrated approaches to methylome analysis.