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Related Experiment Video

Updated: Jun 15, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Electrically assisted capillary liquid chromatography using a silica monolithic column.

Bo Zhang¹, Edmund T Bergström, David M Goodall

¹Department of Chemistry, University of York, York, UK.

Journal of Chromatography. A

|March 12, 2010

Summary

A novel duplex column combines capillary liquid chromatography and electrophoresis for enhanced separations. This electrically assisted capillary liquid chromatography (eCLC) method achieves high speed and resolution for diverse compounds.

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Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

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Published on: February 27, 2026

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Last Updated: Jun 15, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Published on: December 4, 2021

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary liquid chromatography (CLC) and capillary electrophoresis (CE) are powerful separation techniques.
Combining CLC and CE can offer enhanced separation capabilities.
Silica monolithic columns provide excellent permeability for efficient separations.

Purpose of the Study:

To investigate the combination of chromatography and electrophoresis in electrically assisted capillary liquid chromatography (eCLC).
To develop a duplex column integrating a silica monolithic section with an open capillary.
To achieve high-speed and high-resolution separations with tunable selectivity.

Main Methods:

Fabrication of a duplex column by linking a silica monolithic capillary column to an open capillary.
Utilizing a commercial CE instrument with a reversed-phase silica monolithic section.
Employing electrically assisted capillary liquid chromatography (eCLC) mode, varying pressure and voltage.
Implementing step flow gradients for compound elution.

Main Results:

Achieved a minimum plate height of 9 microm in CLC mode at 50 psi.
Demonstrated high-speed and high-resolution separations of acidic and basic compounds in eCLC mode.
Showcased selectivity tuning by adjusting pressure and voltage.
Successfully eluted compounds with different charge states using step flow gradients.

Conclusions:

The developed duplex column and eCLC mode offer a flexible and powerful platform for complex separations.
eCLC provides tunable selectivity through combined pressure and voltage control.
Silica monolithic columns are well-suited for eCLC applications, enabling efficient separations and gradient elution.