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Updated: Jun 15, 2026
08:40
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Published on: October 21, 2022
MRC1-dependent scaling of the budding yeast DNA replication timing program
Amnon Koren1, Ilya Soifer, Naama Barkai
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Genome Research
|March 12, 2010
Summary
Most DNA replication timing mutants maintain relative origin firing order when S phase is extended. However, Mrc1 deletion activates dormant origins, revealing its role in robust replication timing programs.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- DNA replication timing is crucial for genome stability.
- Understanding how S phase duration affects replication timing is essential.
Purpose of the Study:
- To investigate DNA replication timing programs in yeast mutants with extended S phase.
- To identify genes and pathways regulating replication timing robustness.
Main Methods:
- Genome-wide screening to identify yeast mutants with extended S phase.
- Analysis of DNA replication origin activation times in mutant strains.
- Characterization of origin inactivation and dormancy.
Main Results:
- Thirteen of fourteen mutants showed scaled origin activation times with extended S phase.
- Inactive origins in these mutants were small and distributed throughout S phase.
- Mrc1 deletion maintained wild-type origin firing times despite a twofold S phase extension, activating numerous dormant origins.
Conclusions:
- Most S phase perturbations affect the entire replication timing program, maintaining relative origin firing order.
- Mrc1 plays a key role in maintaining the robustness of the DNA replication timing program.
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