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Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

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Integrated AC electrokinetic cell separation in a closed-loop device.

Zachary Gagnon¹, Jill Mazur, Hsueh-Chia Chang

¹Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecualar Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA.

|March 12, 2010

Summary

This study integrates micro-pumps and dielectrophoretic (DEP) traps for rapid yeast cell concentration and separation on-chip. The system utilizes electric fields to manipulate both live and dead yeast cells effectively.

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Area of Science:

Biotechnology
Microfluidics
Cellular Engineering

Background:

On-chip cell manipulation is crucial for biological research and diagnostics.
Existing methods for cell concentration and separation often require complex setups or multiple steps.

Purpose of the Study:

To develop an integrated microfluidic system for simultaneous yeast cell concentration and separation.
To leverage electrothermally induced micro-pumps and dielectrophoretic (DEP) traps for efficient on-chip cell handling.

Main Methods:

Integration of electrothermally induced micro-pumps and DEP traps within micro-circulating fluidic channel loops.
Utilizing embedded electrodes for both fluid transport and cellular manipulation.
Investigating frequency-dependent DEP behavior of viable and non-viable yeast cells.

Main Results:

Demonstrated operating modes using positive DEP (pDEP) and negative DEP (nDEP) for cell concentration and separation.
Achieved rapid concentration of both live and dead yeast cells in high or low electric field regions.
Successfully separated yeast cells by directing them to distinct high-field and low-field regions.

Conclusions:

The integrated microfluidic device enables rapid and efficient on-chip yeast cell concentration and separation.
The system's design allows for versatile cell manipulation by controlling electric field parameters.
This technology offers a promising platform for various cell-based assays and diagnostics.