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Updated: Jun 15, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Genetic characterization of Metapenaeus affinis (H. M. Edwards, 1837) using RAPD markers
W S Lakra1, M Goswami, Akhilesh Singh
1National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkusha, Lucknow, 226002, India. wslakra@gmail.com
Abstract:
Genetic structure of four populations of Metapenaeus affinis from Maharashtra, Orissa, Kerala and Tamil Nadu in India was studied using RAPD markers. Five selective primers provided distinct and consistent RAPD profiles in all the four populations. The bands in the range 225-1,900 bp were scored for consistent results. The RAPD profiles generated by all the five primers revealed varying degrees of polymorphism, ranging from 25.00% (primer E-03) to 65.00% (primer E-06). Nei's (Nei M, Natl Acad Sci Proc USA 70:3321-3323, 1973) genetic diversity (h) among the four populations varied from 0.2565 ± 0.2146 (Orissa population) to 0.3576 ± 0.1897 (Maharashtra population).
