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Related Experiment Video

Updated: Jun 15, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Probing stemness and neural commitment in human amniotic fluid cells.

Anna Jezierski1, Andree Gruslin, Roger Tremblay

  • 1Neurogenesis and Brain Repair, Neurobiology Program, Institute for Biological Sciences, National Research Council Canada, 1200 Montreal Road, Ottawa, Canada.

Stem Cell Reviews and Reports
|March 12, 2010
PubMed
Summary

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Human amniotic fluid (AF) cells offer a promising source for regenerative medicine. Researchers identified stem cell populations within AF, capable of neural differentiation, advancing tissue engineering applications.

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Developmental biology

Background:

  • Human amniotic fluid (AF) cells are explored as an alternative to embryonic stem (ES) cells for transplantation and tissue engineering.
  • AF cells bypass ethical concerns associated with ES cells and avoid lineage commitment issues of adult stem cells.
  • Improved isolation protocols for AF stem cell sub-populations are needed for therapeutic applications.

Purpose of the Study:

  • To investigate molecular factors in AF cell self-renewal, neural commitment, and differentiation.
  • To analyze AF cells from various gestational ages.
  • To identify and characterize stem cell populations within AF.

Main Methods:

  • Morphological and molecular analysis of AF cells.

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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Related Experiment Videos

Last Updated: Jun 15, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

  • Expression analysis of KERATIN 8 (K8), NOTCH signaling components, NESTIN, OCT4a, NANOG, and SOX2.
  • Single cell cloning of SOX2 positive AF cells.
  • Assessment of neuronal differentiation potential and marker expression (MAP2, NFL, NSE).

    • Main Results:

    • Early gestational AF cells predominantly expressed KERATIN 8 (K8), indicating epithelial origin.
    • AF cells showed expression of NOTCH signaling pathway components.
    • A small sub-population (<10%) of K8+ cells co-expressed NESTIN.
    • Fewer than 1% of AF cells expressed pluripotency markers (OCT4a, NANOG, SOX2).
    • SOX2 positive AF clones differentiated into neuron-like cells expressing neuronal markers.

    Conclusions:

    • Amniotic fluid contains fetal cells with stem cell characteristics.
    • These cells possess potential for neural differentiation.
    • Further research is warranted for their biological understanding and clinical utility in regenerative medicine.