Acinetobacter brisouii sp. nov., isolated from a wetland in Korea

A bacterial strain 5YN5-8(T) was isolated from peat layer on Yongneup in Korea. Cells of strain 5YN5-8(T) were strictly aerobic, Gram-negative, coccobacilli, non-spore forming, and non-motile. The isolate exhibited optimal growth at 28 degrees C, pH 7.0, and 0-1% NaCl. Results of 16S rRNA gene sequence analyses indicated a close relationship of this isolate to Acinetobacter calcoaceticus (97.8% similarity for strain DSM 30006(T)). It also exhibited 94.4-97.8% 16S rRNA gene sequence similarities to the validly published Acinetobacter species. The value for DNA-DNA hybridization between strain 5YN5-8(T) and other members of the genus Acinetobacter ranged from 16 to 28%. Predominant cellular fatty acids were C(18:1) omega9c, summed feature 4 containing C(15:0) iso 2-OH and/or C(16:1) omega7c, and C(16:0). The DNA G+C content was 43.9 mol%. Phylogenetic, phenotypic, and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Acinetobacter. The name Acinetobacter brisouii sp. nov. is proposed for the novel species, with 5YN5-8(T) (=KACC 11602(T) = DSM 18516(T)) as the type strain.