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Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jun 15, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Databases of protein-protein interactions and complexes.

Hong Sain Ooi1, Georg Schneider, Ying-Leong Chan

  • 1Bioinformatics Institute, Agency for science, Technology, and Research, Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2010
PubMed
Summary

Genomic information is realized through protein interactions, forming molecular machines. Curated protein-protein interaction (PPI) databases are essential for research, with portals offering unified access and added value.

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Protein Complex Affinity Capture from Cryomilled Mammalian Cells

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Last Updated: Jun 15, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
10:37

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Published on: December 9, 2016

Area of Science:

  • Molecular Biology
  • Bioinformatics

Background:

  • Proteins translate genomic information into functional molecules.
  • Proteins interact physically or indirectly to perform cellular functions.
  • Complex protein assemblies act as molecular machines or structural building blocks.

Purpose of the Study:

  • To review the scope and quality of protein-protein interaction (PPI) databases.
  • To assess the retrieval tools available for PPI data.
  • To highlight portals offering unified access to PPI databases.

Main Methods:

  • Review of existing protein-protein interaction databases.
  • Analysis of database scope and annotation quality.
  • Evaluation of data retrieval tools and access portals.

Main Results:

  • High-throughput techniques have generated large-scale protein interaction data.
  • Curated protein-protein interaction (PPI) databases are crucial for research efficiency.
  • Unified access portals enhance the utility of PPI data.

Conclusions:

  • The availability of curated PPI databases is vital for biological research.
  • Effective retrieval tools and unified portals are necessary for navigating complex PPI data.
  • Further development of databases and access platforms will advance our understanding of protein functions.