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Updated: Jun 15, 2026
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
[Screening and identification of a fungal strain functioning in hydroxylation of puerarin]
Liping Yang1, Lei Xu, Xinyu Zhu
1College of Life Science, Nantong University, Nantong 226007, China. ylp325@tom.com
Objective:
Puerarin is a Chinese herbal medicine. We isolated a fungus from soil which could convert puerarin to 3'-hydroxypuerarin.
Methods:
Strains were cultivated on potato dextrose agar (PDA). The biotransformation product was extracted with an organic solvent and detected on high performance liquid chromatography(HPLC).
Results:
A total of 186 fungal strains were isolated from soil, and strain NT-01 was determined to transform puerarin into 3'-hydroxypuerarin. The product was identified as 3'-hydroxypuerarin by MS and 1H-NMR and 13C-NMR. The morphology characteristic of strain NT-01 was consistent with Gliocladium deliguescens. Phylogenic analysis on sequences showed that strain NT-01 was clustered together with G. deliguescens and G. viride with high boostrap supporting, so that strain NT-01 was identified as Gliocladium sp.
Conclusion:
Gliocladium strain NT-01 can convert puerarin to 3'-hydroxypuerarin.
