Related Experiment Video
Updated: Jun 15, 2026
06:36
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Human lactoferrin stimulates skin keratinocyte function and wound re-epithelialization
1Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
The British Journal of Dermatology
|March 13, 2010
Summary
Human lactoferrin (hLF) significantly enhances wound healing by promoting skin cell proliferation and migration. This study demonstrates hLF
Area of Science:
- Biochemistry
- Cell Biology
- Wound Healing Research
Background:
- Human lactoferrin (hLF) exhibits antimicrobial and anti-inflammatory properties.
- hLF may stimulate cell proliferation in non-skin cell lines.
Purpose of the Study:
- Investigate the role of hLF in promoting wound re-epithelialization.
- Assess the effects of holo-rhLF on keratinocyte behavior and wound healing.
Main Methods:
- Assessed cell growth, migration, attachment, and survival using various assays.
- Utilized pathway inhibitors and RNA interference to explore hLF mechanisms.
- Evaluated wound re-epithelialization in an in vivo swine burn model.
Main Results:
- Holo-rhLF stimulated keratinocyte proliferation and migration via MAPK, Src, and Rho/ROCK pathways.
- Lactoferrin receptor LRP1 is involved in hLF-mediated migration.
- Holo-rhLF increased cell viability and inhibited apoptosis.
- Significantly accelerated wound re-epithelialization in a swine burn model.
Conclusions:
- Holo-rhLF directly enhances wound re-epithelialization by promoting keratinocyte proliferation and migration.
- Holo-rhLF protects cells from apoptosis, contributing to wound healing.
- Demonstrated potential therapeutic applications of hLF in wound healing.
Related Concept Videos
01:28Phases of Wound Repair
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
01:11Healing I: Introduction
Healing is the physiological process by which the body restores the integrity and function of damaged tissues following injury. It involves a coordinated interplay of cellular proliferation, extracellular matrix remodeling, and growth factor signaling. The extent and nature of the tissue damage determine whether healing occurs by resolution, regeneration, or replacement.ResolutionResolution represents the most complete form of healing, occurring when the injury is minimal and tissue...
01:19Clinical Applications of Epidermal Stem Cells
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:24Liver Regeneration
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
01:09Introduction to Fibroblasts
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...