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Updated: Jun 15, 2026
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Published on: March 3, 2017
Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins
Lijun Zhang1, Xiaofang Jia1, Xiaojun Zhang2
1Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Background:
The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.
Results:
26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.
Conclusions:
Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.
Insights
This study investigated protein changes in peripheral blood mononuclear cells (PBMCs) from HIV-1 patients, identifying altered protein expression and potential biomarkers for HIV research.
Area of Science:
- Proteomics
- Virology
- Immunology
Background:
- The human immunodeficiency virus type 1 (HIV-1) pandemic remains a significant global health challenge.
- Understanding HIV-1's impact on host cells is crucial for developing effective interventions.
Purpose of the Study:
- To perform differential proteome research on peripheral blood mononuclear cells (PBMCs) from HIV-1 positive patients and healthy controls.
- To identify host proteins and potential biomarkers affected by HIV-1 infection.
Main Methods:
- Differential proteome analysis using two-dimensional electrophoresis (2DE).
- Validation of protein expression using real-time RT-PCR and Western blotting.
- Bioinformatic analysis to assess host protein network disruption.
Main Results:
- Identified 12 up-regulated and 1 down-regulated proteins in HIV-1 positive patients compared to controls.
- Confirmed up-regulation of talin-1, vinculin, and coronin-1C mRNA expression.
- Detected fragments of vinculin, talin-1, and filamin-A in HIV-1 positive samples, indicating disrupted protein networks.
Conclusions:
- The study provides insights into HIV-1 and host cell protein interactions.
- Discovered potential novel biomarkers, including fragments of vinculin, filamin-A, and talin-1, for anti-HIV research.
