Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bioactive Magnesium Silicate Activating Myocardial Energy Metabolism For Infarcted Myocardium Repair.

Exploration (Beijing, China)·2026

Same author

Specific Multimodal Imaging of Deep-Seated Tumor with High Intratumoral Retention <i>via In Situ</i> Assembly of Probes.

ACS nano·2026

Same author

Wash-Free β-Galactosidase-Activated Fluorescent Probe for Assessing Chemotherapy Efficacy in Ovarian Cancer Organoids.

Analytical chemistry·2026

Same author

Efficacy and safety of cyclosporine plus luspatercept versus cyclosporine in newly diagnosed non-transfusion-dependent non-severe aplastic anemia: A prospective randomized trial.

BMC medicine·2026

Same author

Design and evaluation of a dicationic ionic liquid-embedded C18 stationary phase for mixed-mode chromatography.

Journal of chromatography. A·2026

Same author

Charge-Regulation Activable Theranostic Probe for Tumor-Specific Fluorescence Imaging and Chemo-Phototherapy.

Advanced healthcare materials·2026

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Novel luminescent nanoparticles for DNA detection.

Ling Dong¹, Zhihua Yang, Yi Zhang

¹College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|March 13, 2010

Summary

Highly luminescent lanthanum fluoride (LaF3) nanocrystals were developed for detecting trace amounts of nucleic acids. This simple fluorescence quenching method offers a rapid and reliable approach for DNA quantification.

More Related Videos

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Related Experiment Videos

Last Updated: Jun 15, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Area of Science:

Materials Science
Nanotechnology
Analytical Chemistry

Background:

Lanthanum fluoride (LaF3) nanocrystals doped with cerium (Ce3+) and terbium (Tb3+) exhibit strong luminescence.
Surface functionalization of nanomaterials is crucial for their application in biological systems.
Nucleic acids can interact with and quench the luminescence of certain nanomaterials.

Purpose of the Study:

To prepare and characterize highly luminescent LaF3:Ce3+/Tb3+ nanocrystals.
To develop a facile fluorescence quenching method for nucleic acid detection.
To evaluate the sensitivity and reliability of the developed method for DNA quantification.

Main Methods:

Layer-by-Layer (LbL) technology for surface functionalization of LaF3:Ce3+/Tb3+ nanocrystals.
Preparation of stable and dispersible nanocrystals in aqueous solutions.
Development of a fluorescence quenching assay for nucleic acid detection.

Main Results:

Successfully synthesized highly luminescent and photostable LaF3:Ce3+/Tb3+ nanocrystals.
Demonstrated efficient luminescence quenching by nucleic acids.
Established a linear relationship between fluorescence intensity and DNA concentration for calf thymus DNA (ct-DNA) and herring sperm DNA (hs-DNA).
Achieved low detection limits for both ct-DNA (0.21 µg/mL) and hs-DNA (0.31 µg/mL).

Conclusions:

The developed method is simple, rapid, and exhibits a wide linear range for DNA detection.
The functionalized nanocrystals are suitable for sensitive nucleic acid quantification in aqueous solutions.
The method shows satisfactory recovery and precision, indicating its potential for practical applications.