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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 15, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

Aneuploidy detection in mixed DNA samples by methylation-sensitive amplification and microarray analysis.

Lucia Brown¹, Gregory Brown, Pamela Vacek

¹Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT 05405, USA.

Clinical Chemistry

|March 13, 2010

Summary

This summary is machine-generated.

A new DNA methylation analysis method enables noninvasive detection of fetal aneuploidy from small plasma samples. This technique accurately identifies chromosomal abnormalities, overcoming challenges posed by limited fetal DNA quantities.

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Last Updated: Jun 15, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

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Published on: May 6, 2022

Single Oocyte Bisulfite Mutagenesis

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Published on: June 27, 2012

Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Area of Science:

Genetics
Molecular Biology
Prenatal Diagnostics

Background:

Cell-free fetal nucleic acid in maternal plasma is limited for genetic testing due to low quantity and high maternal DNA contamination.
Detecting fetal aneuploidy noninvasively requires methods robust to these challenges.

Purpose of the Study:

To develop and evaluate a novel method for noninvasive fetal aneuploidy detection.
To assess the utility of methylation-sensitive amplification and microarray analysis for analyzing small fetal DNA samples.

Main Methods:

Developed methylation-sensitive amplification for small DNA samples (approx. 1 ng).
Utilized 2-color microarray analysis with a custom array to assess relative DNA methylation.
Applied the method to mixtures of placental DNA and maternal blood DNA.

Main Results:

Microarray assessment of genomic methylation correlated with bisulfite-conversion PCR.
First-trimester trophoblast DNA was generally hypomethylated compared to whole blood DNA.
Microarray signals differed significantly (P < 0.001) for trisomic chromosomes in spiked samples.

Conclusions:

The developed method shows potential for noninvasive detection of fetal aneuploidy.
This approach addresses limitations of small and contaminated fetal DNA samples in maternal plasma.