K+ channels on resting duct cells from rat pancreas

The ductal system of the exocrine pancreas produces HCO(3)(-)-rich fluid in response to secretin and other stimuli. HCO(3)(-) efflux across the luminal membrane is mediated by a Cl(-)-HCO(3)(-) exchanger operating in parallel with the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel. Basolateral K(+) channels provide an exit pathway for K(+) and play a vital role in maintaining the membrane potential, which is a crucial component of the driving force for anion secretion. Measurements of membrane potential with intracellular microelectrodes suggested that Ba(2+)-sensitive K(+) conductance accounts for more than 60% of the total basolateral ionic conductance in resting ducts (1). To identify the Ba(2+)-sensitive K(+) channels, we isolated ducts from normal rat pancreas by collagenase digestion. We first demonstrated that the ducts did not express a vascular endothelial marker PECAM-1 (platelet/endothelial cell adhesion molecule-1), but expressed cytokeratin 20, a marker of duct cells (2), using immunofluorescent staining. In addition, monoclonal anti-CFTR antibody was detected near the luminal membrane of these cells. In cell-attached single-channel recordings, we observed three types of K(+) channels on basolateral membrane in unstimulated duct cells. The 40 pS K(+) channels are likely to mediate whole-cell inwardly rectifying K(+) (Kir) currents, which were blocked by extracellular Ba(2+) in a voltage-dependent manner. The properties of 90 pS and 170 pS K(+) channels are similar to those of Ca(2+)-activated K(+) channels. We then identified Kir2.0 and SK4/IK1 (intermediate conductance Ca(2+)-activated K(+) channel) subunits as molecular candidates of the K(+) channels using RT-PCR analysis. The present results suggest that these subunits may mediate native K(+) currents in resting duct cells. Further functional studies with specific blockers are required to evaluate which of these K(+) channels contribute to the resting membrane potential and might be involved in HCO(3)(-) secretion.