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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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[Dip-slides for culturing urine in general practice].

Geir Thue¹, Anders Baerheim, Wenche Iren Bjelkarøy

¹NOKLUS, Postboks 6165, 5892 Bergen og Seksjon for allmennmedisin, Institutt for samfunnsmedisinske fag, Universitetet i Bergen. geir.thue@isf.uib.no

Tidsskrift for Den Norske Laegeforening : Tidsskrift for Praktisk Medicin, Ny Raekke

|March 13, 2010

Summary

The new lower threshold for significant bacteriuria (103 CFU/ml) makes dip-slides less reliable for uncomplicated urinary tract infections. Proper urine sampling and handling are crucial for accurate diagnosis.

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Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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Published on: August 20, 2021

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

Published on: May 8, 2021

Area of Science:

Microbiology
Clinical Diagnostics
Urology

Context:

A recent consensus meeting lowered the threshold for significant bacteriuria to 103 colony-forming units (CFU)/ml.
This new threshold poses challenges for the accurate interpretation of dip-slide tests in primary care settings.
The Norwegian quality improvement of laboratories in primary care (NOKLUS) initiated a review of dip-slide utility.

Purpose:

To review the use of dip-slides in general practice in light of the revised significant bacteriuria threshold.
To evaluate the suitability of dip-slides for diagnosing various urinary tract infections (UTIs).
To provide guidance on optimal urine sample collection and handling.

Summary:

Escherichia coli is the predominant pathogen in UTIs and asymptomatic bacteriuria in pregnant women.
Bacterial concentrations in most UTIs, especially upper UTIs, are typically ≥104 CFU/ml.
Dip-slides are suitable for urine transport exceeding two days; otherwise, boric acid preserved samples are preferred.

Impact:

Dip-slides with E. coli-agar can aid in diagnosing complicated UTIs and asymptomatic bacteriuria in pregnant women.
Dip-slides are not recommended for uncomplicated cystitis.
Healthcare providers must ensure expertise in dip-slide use and participate in quality assurance programs, emphasizing proper urine sampling and handling due to the lower bacteriuria threshold.