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Related Concept Videos

Forced Transdifferentiation01:28

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Jun 15, 2026

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
14:22

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Published on: July 16, 2011

Manipulating the cell differentiation through lentiviral vectors.

Valeria Coppola1, Cesare Galli, Maria Musumeci

  • 1Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore Sanità, Rome, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2010
PubMed
Summary

This study details a method using lentiviral vectors to isolate pure cardiomyocytes from mouse embryonic stem cells. This technique aids in developing new treatments for degenerative diseases and cancer therapy side effects.

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Published on: March 29, 2019

Area of Science:

  • Stem cell biology
  • Cardiovascular research
  • Gene therapy

Background:

  • Cell differentiation is crucial for regenerative medicine and treating diseases like cancer.
  • Generating pure cell populations is essential for effective cell-based therapies.

Purpose of the Study:

  • To describe a method for obtaining a pure cardiomyocyte lineage from murine embryonic stem cells (mES).
  • To demonstrate the utility of tissue-specific promoter lentiviral vectors for cell lineage selection.

Main Methods:

  • Utilized a lentiviral vector system with a cardiac troponin promoter to drive gene expression in mES.
  • Co-infected cells with vectors expressing EGFP (for transduction monitoring) and puromycin resistance (for selection).
  • Applied a selection strategy based on the cardiac troponin promoter's specificity.

Main Results:

  • Successfully isolated a pure population of cardiomyocytes.
  • Demonstrated the efficiency of the lentiviral vector system for targeted cell selection.
  • Validated the use of the cardiac troponin promoter for cardiomyocyte lineage specification.

Conclusions:

  • The described method provides a robust approach for generating pure cardiomyocyte populations.
  • This technique is adaptable for isolating other specific cell lineages from stem cells.
  • Offers a valuable tool for regenerative medicine and disease modeling.