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Related Concept Videos

RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

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Related Experiment Video

Updated: Jun 15, 2026

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
10:52

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters

Published on: May 29, 2026

Solution structure of the Drosha double-stranded RNA-binding domain.

Geoffrey A Mueller1, Matthew T Miller1, Eugene F DeRose1

  • 1Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.

Silence
|March 16, 2010
PubMed
Summary

The nuclear RNase III enzyme Drosha

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CD Spectroscopy to Study DNA-Protein Interactions
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Related Experiment Videos

Last Updated: Jun 15, 2026

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
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Published on: May 29, 2026

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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CD Spectroscopy to Study DNA-Protein Interactions
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CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Drosha is a nuclear RNase III enzyme crucial for microRNA processing.
  • It forms the Microprocessor complex with DGCR8, cleaving primary microRNA.
  • Drosha possesses two RNase III catalytic domains and a dsRBD.

Purpose of the Study:

  • To elucidate the function of Drosha's C-terminal dsRBD.
  • To determine the nuclear magnetic resonance (NMR) solution structure of Drosha-dsRBD.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Determination of the solution structure of Drosha-dsRBD.

Main Results:

  • The solution structure of Drosha-dsRBD was determined.
  • It exhibits an alphabetabetabetaalpha fold common to dsRBDs.
  • A unique extended loop differentiates it from other known dsRBD structures.

Conclusions:

  • The Drosha-dsRBD structure suggests retained RNA-binding capabilities.
  • This domain may play a role in substrate recognition within the Drosha-DGCR8 Microprocessor complex.