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Updated: Jun 15, 2026

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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum
Koji Dohi1, Junko Isoyama-Tanaka, Toru Tokuda
1International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Journal of Bioscience and Bioengineering
|March 16, 2010
Abstract:
The C-terminal catalytic domain of tobacco N-acetylglucosaminyltransferase I fused to maltose-binding protein was produced in Escherichia coli as a soluble form with significant activity. The protein was affinity-purified using amylose resin, and its enzymatic properties were investigated, including its divalent cation requirements, optimal temperature, optimal pH, and substrate specificity.

